Last wood igg4/8/2023 The detection of specific IgG 3 did not correlate with the presence of specific IgM. Thus for the asymptomatic patient for whom other serological tests suggest a recent rubella infection, the failure to detect specific IgG 3 in sequential sera collected after contact suggests reinfection rather than primary rubella. However, only one of 9 sera collected 30–50 days after contact contained specific IgG 3. Of the remaining 19 cases, 7 had detectable specific IgG 3. In 2 of the 21 cases of asymptomatic reinfection only a very early or a very late serum was available. In three cases of symptomatic reinfection, specific IgG 3 was detectable in two but not in the remaining case. Sera from 24 cases of rubella reinfection were examined and all contained specific IgG 1. One case had become negative for specific IgG 3 by day 56. Serum taken on day 21 from one case was negative for specific IgG 3 but the absence of later sera precluded further investigation. Specific IgG 3 became detectable in all cases except one by day 16. In 79 cases of primary rubella, specific IgG 1 developed in all cases by day 8. All 63 sera containing > 15 international units rubella antibody by RH from cases of rubella in the remote past contained specific IgG 1 and eight contained specific IgG 3. None of these 130 was positive for specific IgG 3. The sensitivity of the assay for specific IgG 1 was confirmed by examining 25 selected sera negative by RH but reactive by LA. Of 105 unselected sera negative for rubella antibody by radial haemolysis (RH), two gave concentrations of specific IgG 1 > 3 au and both were positive by rubella latex agglutination (LA). No sera reactive for specific IgG 2 and IgG 4 have been found, and thus the assay reagents were controlled by testing dilutions of a standard calibrant serum containing known concentrations of the specific IgG subclasses. A concentration of 3 au was taken as that indicating positivity for specific IgG 1 and specific IgG 3. For rubella-specific IgG 1 and IgG 3 sera were quantitated in arbitrary units (au) by comparison with standard curves. A solid-phase antigen enzyme-linked immunosorbent assay (ELISA) was developed for the detection of rubella-specific IgG subclasses.
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